Microscopy - recent publications

Endothelial and hematopoietic hPSCs differentiation via a hematoendothelial progenitor Alejandra Vargas‑Valderrama, Anne‑Charlotte Ponsen, Morgane Le Gall, Denis Clay, Sébastien Jacques, Tudor Manoliu, Valérie Rouffiac, Karine Ser‑le‑Roux, Cyril Quivoron, Fawzia Louache, Georges Uzan, Maria‑Teresa Mitjavila‑Garcia, Estelle Oberlin1† and Hind Guenou

Cytometry and Imaging - Microscopy : equipment

Microscopy - Equipments

SP8 Confocal Microscope (Leica)

The system in reverse configuration is equipped with 4 lasers covering excitation wavelengths 405, 488, 561, and 638nm. Fluorescence signals are collected on 2 PMT detectors and 2 high-sensitivity HyD detectors. A thermostatic and CO2-regulated chamber and a motorized turntable are installed on the microscope for kinetic studies and XYZT acquisitions.

This microscope is suitable for confocal, wide-field and/or multi-position imaging.

Cytometry and Imaging - Microscopy : Activities

back to job offer list

back to clinical trials

Microscopy - Activities

Theoretical classes and practical training in photonic microscopy technics
Confocal and multiphoton imaging of cells and tissues
TIRF, live-SR, FRAP, FRET, Photoconversion, Optogenetics
Multidimensional dynamic imaging in living organs & 3D organoid models
Support for the development of new optical devices
Design of experimental procedures
Scientific animations
Technological developments and innovation
Imaging of organoid models

Cytometry & Mass imaging - Team members

back to job offer list

back to clinical trials

Cytometry & Mass imaging - Team members

Operational manager:
Philippe Rameau (Research Engineer)
Tel. 01.42.11.51.38
philippe.rameau@gustaveroussy.fr

Cyril Catelain (Research Engineer)
Tel. 01.42.11.51.38
cyril.catelain@gustaveroussy.fr

Cytometry & Mass imaging - Publications

back to job offer list

back to clinical trials

Cytometry & Mass imaging - Publications

Low Doses of Radiation Increase the Immunosuppressive Profile of Lung Macrophages During Viral Infection and Pneumonia Meziani L, Robert C, Classe M, Da Costa B, Mondini M, Clémenson C, Alfaro A, Mordant P, Ammari S, Le Goffic R, Deutsch E.. Int J Radiat Oncol Biol Phys. 2021 Aug 1;110(5):1283-1294. doi: 10.1016/j.ijrobp.2021.03.022. Epub 2021 Mar 13. PMID: 33722770; PMCID:PMC7954779.

Cytometry & Mass imaging - Equipments

back to job offer list

back to clinical trials

Cytometry & Mass imaging - Equipments

Mass cytometer HELIOS (Fluidigm)

As a complementary tool to flow cytometers, the mass cytometer considerably increases the number of parameters that can be analyzed (>40), while eliminating the problems of overlapping fluorescence spectrums observed with conventional cytometry.

Cytometry and Mass imaging - Activities

back to job offer list

back to clinical trials

Cytometry & Mass imaging - Activities

Training and coaching

Flow, spectral and mass cytometry
Cell sorting and single-cell isolation
Mass imaging for molecular, cellular and functional spatial mapping of cells and tissues
Bioinformatics adapted to the unsupervised analysis of cytometry and imaging data.

Design of experimental procedures

Research in Immunotherapy

back to job offer list

back to clinical trials

PREMIS: A programme dedicated to immunotherapy toxicity

By stimulating patients' immune systems, immunotherapy drugs can generate autoimmune side effects, especially when used in combination, which can affect several organs, sometimes irreversibly. Yet there are no biomarkers to identify patients at risk of developing severe complications. Their discovery is a major objective in improving the management and safety of treated patients.